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Murine acinar pancreatitis preceding necrotizing myocarditis after coxsackievirus B3 inoculation
Author(s) -
Gómez Ricardo M.,
Lascano Eduardo F.,
Berrìa Marìa I.
Publication year - 1991
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890350202
Subject(s) - coxsackievirus , myocarditis , necrosis , viral myocarditis , basophilic , pathology , pancreas , weanling , virus , periodic acid–schiff stain , biology , pancreatitis , viral replication , virology , enterovirus , staining , medicine , endocrinology
Balb/C weanling mice were inoculated intraperitoneally with a myocarditic variant of coxsackie virus B3, with the aim of characterizing more fully the cell damage induced in the heart as well as in other organs. During the first week postin‐fection (pi), all animals developed acinar pancreatitis, followed by focal myocarditis. In accordance with the increasing infectivity titers, such progressive histopathological changes correlated with local viral replication. From day 4 pi, acinar degeneration accompanied by diffuse inflammatory exudate was observed in the pancreas, followed by fatty tissue replacement by day 8. In the heart, focal necrosis rather than inflammatory reaction first appeared at 4 days pi and became widespread by 6–8 days pi. Necrotic foci usually presented calcium deposits, with absence of myofibrils in the affected fibers. The fact that both periodic acid Schiff (PAS) and Best carmine staining remained positive even after diastase treatment ruled out basophilic necrosis. In summary, the pancreas appeared to be the site of primary viral replication leading to viremia.