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Removal of parvovirus B19 from contaminated factor VIII during fractionation
Author(s) -
Schwarz Tino F.,
Roggendorf Michael,
Hottenträger Barbara,
Stolz Wilhelm,
Schwinn Horst
Publication year - 1991
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890350107
Subject(s) - parvovirus , parvoviridae , virology , fractionation , contamination , chemistry , biology , virus , microbiology and biotechnology , chromatography , ecology
A solution of pooled cryo‐precipitate for preparing factor Vlll (F VIII) by the solventi/detergent method was contaminated experimentally with parvovirus B19‐positive plasma to evaluate virus reduction achieved by the final steps of the F Vlll production process. Virus reduction was at least 2 logs of the total amount of B19 virus added to the pooled cryo‐precipitate. The major amount of B19 virus was detected in the solution used to regenerate the F VIII‐selective anion exchange chromatography column. A few viral particles were detected in the final F Vlll concentrate before filtration but these were aggregates and were removed by filtration, and in the solution used to regenerate the anion exchange resin. It is not known whether the residual viral DNA present in the final product represents infectious or inactivated particles.