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Recombinant immunoblot assay for hepatitis C antibody in patients with posttransfusion non‐A, non‐B hepatitis
Author(s) -
Wang JinTown,
Wang TehHong,
Lin JawTown,
Sheu JinChuan,
Lin SyMing,
Sung JueiLow,
Chen DingShinn
Publication year - 1991
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890340307
Subject(s) - medicine , antibody , recombinant dna , virology , hepatitis , indeterminate , hepatitis c , viral disease , hepatitis c virus , immunology , biology , virus , biochemistry , mathematics , gene , pure mathematics
In a prospective study of 287 patients who received blood transfusion, 26 who were found positive for hepatitis C antibody (anti‐HCV) by an enzyme‐linked immunosorbant assay (ELISA) were studied by a recombinant immunoblot assay (RIBA). Nineteen of the 26 patients had posttransfusion non‐A, non‐B (NANB) hepatitis. Sixteen (84.2%) of the 19 patients with hepatitis had positive results by RIBA, 2 had indeterminate results, and 1 was negative. By contrast, five of the 7 recipients without hepatitis were negative, 1 indeterminate, and 1 positive by RIBA. Those with negative RIBA results had significantly lower optical density (OD) readings by ELISA than those with positive RIBA tests. Therefore, patients without hepatitis or lower OD value have a higher false‐positive rate in anti‐HCV ELISA than did those with a high OD value or with evidence of hepatitis.