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Preparation of monoclonal antibodies to JC virus and their use in the diagnosis of progressive multifocal leucoeneephalopathy
Author(s) -
Knowles W. A.,
Sharp I. R.,
Efstratiou L.,
Hand J. F.,
Gardner S. D.
Publication year - 1991
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890340211
Subject(s) - jc virus , progressive multifocal leukoencephalopathy , virology , monoclonal antibody , virus , titer , immunofluorescence , antibody , antigen , bk virus , biology , brain biopsy , medicine , immunology , pathology , biopsy , kidney transplantation , kidney , endocrinology
Seven monoclonal antibodies (MAbs) to polyomavirus JC were produced, and one was selected for use in immunofluorescence (IF) tests on brain material from patients with suspected progressive multifocal leucoencephalopathy (PML). The selected MAb (5. 12.2) reacted by IF with JC‐infected primary human foetal glial (PHFG) cell cultures (titre 1/200,000) but not with BK‐infected human embryo lung (HEL) fibroblasts (titre <1/20). Its haemagglutination‐inhibition (HI) titre was high (>1/5 × 10 6 ) against JC virus but low (<1/5)against BK virus. A diagnosis of PML was confirmed on brain biopsy or at postmortem in four patients, two of whom were also infected with human immunodeficiency virus (HIV). In one of the patients, specific detection of JC virus antigen had not been possible using our routine high titred JC and BK human sera due to interference by JC antibody present in the cerebrospinal fluid (CSF). Viral antigen was, however, detected with the MAb 5. 12.2.