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Demonstration of concurrent dengue 1 and dengue 3 infection in six patients by the polymerase chain reaction
Author(s) -
Laille Manola,
Deubel Vincent,
SainteMarie François Flye
Publication year - 1991
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890340109
Subject(s) - dengue fever , virology , dengue virus , polymerase chain reaction , viremia , serotype , biology , monoclonal antibody , immunofluorescence , polymerase , virus , antibody , dna , immunology , gene , genetics
A dual viremia resulting from naturally acquired dengue 1 and dengue 3 infections in six patients experiencing a dengue‐like syndrome during the epidemic in New Caledonia in 1989 is reported. Serotype identification was first based on virus isolation in mosquito cells and immunofluorescence using type‐specific monoclonal antibodies. The double infection was confirmed directly in blood samples by the polymerase chain reaction (PCR) on genomic RNA and hybridization of the amplified cDNA fragments with type‐specific DNA probes.