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Typing and molecular characterization of human papillomaviruses in genital warts from South African women
Author(s) -
Johnson Jennifer E.,
Dehaeck Catherine M. C.,
Soeters Robbert,
Williamson AnnaLise
Publication year - 1991
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890330108
Subject(s) - restriction enzyme , virology , southern blot , genital warts , typing , papillomaviridae , biology , condyloma acuminatum , dot blot , human papillomavirus , sex organ , hpv infection , virus , dna , medicine , genetics , cancer , cervical cancer , syphilis , human immunodeficiency virus (hiv)
Condyloma acuminata from 12 women were examined for the presence of human papilloma‐virus DNA. Six of the women had HPV 6a, two had HPV 11a, one had a new HPV 6 subtype, and one a new HPV 11 subtype. The new HPV 6 subtype could be distinguished from other HPV 6 subtypes on the basis of Pst 1 fragments as well as Hind II fragments. The new HPV 11 subtype had a different Hpa 11 restriction endonuclease pattern. Restriction maps of both new subtypes were constructed. Two of the biopsies did not contain detectable HPV DNA when probed with HPV 6, HPV 11, and HPV16. Biopsies were taken from normal tissue 1 cm away from the condyloma in 11 of the patients. Only one of these normal tissue biopsies was positive for HPV by Southern blot hybridization.