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Sensitive enzyme‐linked immunosorbent assay for antibody to varicella‐zoster virus using purified VZV glycoprotein antigen
Author(s) -
Wasmuth Edward H.,
Miller William J.
Publication year - 1990
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890320310
Subject(s) - antigen , varicella zoster virus , virology , glycoprotein , seroconversion , antibody , virus , biology , immune system , affinity chromatography , enzyme , immunology , microbiology and biotechnology , biochemistry
The development of an ELISA of increased sensitivity has permitted a more critical evaluation of human humoral immune responses to the live attenuated varicella (Oka/Merck) vaccine. For use as a solid‐phase antigen, the glycoprotein (gp) antigens are prepared by lectin‐affinity chromatography from lysates of VZV‐infected MRC‐5 cells. The lot‐to‐lot variation in VZV gp content is controlled by standardization of antigen against a panel of human serum providing antigencoated plates of consistent quality. The increased sensitivity of the gpELISA over the VAR ELISA is reflected in the greater seroconversion rate and prepositive rate specificity. These determinations have been shown to be specific for anti‐VZV by absorption experiments using purified VZV gp antigens.