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5‐bromodeoxyuridine enhancement of Inoue‐Melnick virus synthesis in human colon carcinoma cell lines
Author(s) -
Inoue Y. K.,
Nishibe Y.,
Kimura T.
Publication year - 1990
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890310402
Subject(s) - bromodeoxyuridine , virology , clone (java method) , titer , biology , cell culture , virus , microbiology and biotechnology , staining , immunohistochemistry , dna , immunology , biochemistry , genetics
Inoue‐Melnick virus (IMV) synthesis in naturally infected human colon carcinoma cell lines can be enhanced by 5‐bromodeoxyurdine (BUDR). The optimal conditions for BUDR enhancement differed between two cell lines, SW‐480 and DLD‐1, SW‐480 cells produced 100 times more infectious IMV than DLD‐1 cells by BUDR treatment. A clonal difference in IMV synthesis in SW‐480 cells was also observed. The highest titer of virus was found in the culture fluid of clone H (SW‐480/H) cells treated with BUDR, and a herpes‐like virus was detected by negativestaining electron microscopy of concentrates of the culture fluid. One nonproducer clone (SW‐480/B) was also activated to produce virus when treated with BUDR.