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Characterization of a nucleic acid probe for the diagnosis of human coronavirus 229E infections
Author(s) -
Myint S.,
Harmsen D.,
Raabe T.,
Siddell S. G.
Publication year - 1990
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890310216
Subject(s) - nucleic acid , complementary dna , rna , virology , biology , microbiology and biotechnology , coronavirus , nucleic acid thermodynamics , gene , hybridization probe , chemistry , covid-19 , biochemistry , disease , pathology , infectious disease (medical specialty) , medicine
A cDNA copy of the HCV229E nucleocapsid protein gene was isolated and characterized. Sequence analysis predicts a nucleocapsid polypeptide of 389 amino acids with a molecular weight (mol. wt.) of 43,450. Single strand RNA probes derived from the cDNA copy hybridize specifically to HCV229E RNA and approximately 50 pg of intracellular viral RNA can be readily detected. The application of nucleic acid hybridization as a routine procedure for the diagnosis of HCV229E infection is discussed.