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A rapid and simplified micromethod for subtyping varicella‐zoster virus
Author(s) -
Gharabaghi Farhad,
Aymard Michèle,
Trotemann Pierre,
Gerdil Catherine
Publication year - 1990
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890310210
Subject(s) - subtyping , virology , virus , varicella zoster virus , biology , restriction enzyme , strain (injury) , dna , genetics , anatomy , computer science , programming language
A simplified and rapid micromethod based on restriction endonuclease analysis of radiolabelled varicella‐zoster virus (VZV) DNA is described and applied to strains for comparison. This procedure is cheaper and less time consuming than that requiring viral nucleocapsid purification (macromethod). The micromethod is suitable for routine DNA analysis of VZV isolates, allows differentiation of vaccine strain from wild strains, and provides evidence for variability of wild strains.