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Detection of human coronavirus 229E in nasal washings using RNA:RNA hybridization
Author(s) -
Myint S.,
Siddell S.,
Tyrrell D.
Publication year - 1989
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890290113
Subject(s) - virology , rna , coronavirus , biology , coronaviridae , covid-19 , microbiology and biotechnology , gene , medicine , genetics , disease , pathology , infectious disease (medical specialty)
A method is described for the detection of human coronavirus 229E (HCV 229E) in nasal washings using RNA:RNA filter hybridization. Volunteers were inoculated with HCV 229E, and daily nasal washings were collected. These washings were then examined for the presence of viral RNA using a single‐stranded RNA probe. Nucleic acid hybridization is shown to be a sensitive technique for the diagnosis of HCV 229E infections.