Detection of human coronavirus 229E in nasal washings using RNA:RNA hybridization | Zendy

Myint S. | Zendy; Siddell S. | Zendy; Tyrrell D. | Zendy

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Detection of human coronavirus 229E in nasal washings using RNA:RNA hybridization

Author(s) -

Myint S.,

Siddell S.,

Tyrrell D.

Publication year - 1989

Publication title -

journal of medical virology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.782

H-Index - 121

eISSN - 1096-9071

pISSN - 0146-6615

DOI - 10.1002/jmv.1890290113

Subject(s) - virology , rna , coronavirus , biology , coronaviridae , covid-19 , microbiology and biotechnology , gene , medicine , genetics , disease , pathology , infectious disease (medical specialty)

A method is described for the detection of human coronavirus 229E (HCV 229E) in nasal washings using RNA:RNA filter hybridization. Volunteers were inoculated with HCV 229E, and daily nasal washings were collected. These washings were then examined for the presence of viral RNA using a single‐stranded RNA probe. Nucleic acid hybridization is shown to be a sensitive technique for the diagnosis of HCV 229E infections.

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