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DNA dot‐blot hybridization implicates human papillomavirus type 11‐DNA in epithelioma cuniculatum
Author(s) -
Knobler R. M.,
Schneider S.,
Neumann R. A.,
Bodemer W.,
Radlwimmer B.,
Aberer E.,
SöltzSzöts J.,
Gebhart W.
Publication year - 1989
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890290107
Subject(s) - dot blot , dna , epithelioma , southern blot , biology , virology , nucleic acid thermodynamics , plasmid , dna–dna hybridization , hybridization probe , virus , microbiology and biotechnology , carcinoma , genetics , base sequence
Epithelioma cuniculatum is a rare, low‐grade squamous cell carcinoma, belonging to the group of verrucous carcinomas. Evidence is presented that suggests that human papilloma virus type‐11 may be involved in the pathogenesis of epithelioma cuniculatum. HPV‐DNA was detected by dot‐blot hybridization with 32 P‐labelled probe DNA. Plasmids containing HPV‐DNA were isolated by the benzoylated naphthoylated DEAE cellulose method (BND‐method), an improved procedure for routine detection of HPV‐DNA.