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Hiv genome in peripheral blood mononuclear cells of seronegative regular sexual partners of hiv‐infected subjects
Author(s) -
Pezzella M.,
Vonesch N.,
Sorice F.,
Mannella E.,
Mirolo M.,
Miceli M.,
Rosci M. A.,
Morace G.,
Rapicetta M.,
Macchi B.,
Angeloni P.
Publication year - 1989
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890280402
Subject(s) - peripheral blood mononuclear cell , virology , biology , in situ hybridization , virus , hiv antigens , genome , lentivirus , antibody , complementary dna , human immunodeficiency virus (hiv) , viral disease , immunology , messenger rna , gene , genetics , in vitro
We have investigated the presence of the human immunodeficiency virus (HIV) by using in situ hybridization on peripheral blood mononuclear cells (PBMCs) from seronegative regular sexual partners of HIV‐infected subjects. The cells were hybridized with a 9 kilobase (kb) Sstl‐Sstl lambda BH 10 probe, which was able to recognize both viral mRNA and proviral cDNA. Labeling was done by chemical insertion of an antigenic sulfone group in cytosine moieties and was visualized by a double‐antibody immunohistochemical reaction. In all the subjects studied, the HIV genome was present. The HIV infected cells showed morphological aspects consistent with that of lymphocytes and monocytes. Our data suggest that the anti‐HIV seronegative individuals who are regular sexual partners of HIV‐infected subjects may be HIV‐infected.