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Characterization of two genome types of adenovirus type 31 isolated in stockholm during 1987
Author(s) -
Johansson Mats E.,
Brundin Marianne,
Adamson Lisbeth,
Grillner Lena,
Landqvist Margareta,
Thörner Årke,
Wirgart Benita Zweygberg
Publication year - 1989
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890280202
Subject(s) - restriction enzyme , biology , virology , feces , genome , dna , mastadenovirus , molecular epidemiology , isolation (microbiology) , hindiii , microbiology and biotechnology , genetics , adenoviridae , gene , genotype , recombinant dna
The authors isolated during 1987 seven adenovirus type 31 (Ad31) within a 9‐month period. The isolates were obtained from urine, throat, and feces, implying a systemic spread of the infection. Most patients displayed gastrointestinal symptoms, but some had respiratory symptoms and fever. All of the strains differed by restriction endonuclease analysis from the prototype strain (1315) by an additional BgI II restriction site. Ad31 isolates 1–6 could be divided into two groups by the enzymes Bam HI, Msp I, and Xho I. Each enzyme gave rise to the same group distribution: isolates 1–3 and 4–6, respectively. Digestion with Bst EII, Hind III, Kpn I, and Sma I resulted in identical patterns for isolates 1–6. Isolate 7, however, demonstrated a DNA deletion of approximately 0.8 kbp, but it was otherwise identical to isolates 4–6. In conclusion, two separate genome types of Ad31 were isolated, one of which included a DNA deletion mutant. The increased isolation rate may reflect an epidemiological situation, as the same isolation procedure had been used both before and after this period.