Premium
Detection and typing of human papillomavirus infection of the uterine cervix by dot blot hybridisation: Comparison of scrapes and biopsies
Author(s) -
Hallam Nicholas,
Gibson Patricia,
Green Jonathan,
Charnock Mark
Publication year - 1989
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890270411
Subject(s) - dot blot , virology , uterine cervix , typing , human papillomavirus , cervix , papillomaviridae , medicine , biology , pathology , microbiology and biotechnology , cervical cancer , gene , carcinoma , cancer , genetics
Paired scrapes and biopsies from 100 women attending a routine colposcopy clinic were examined by dot blot DNA hybridisation for infection with human papillomavirus (HPV) types 6/11,16,18, and 31;51% of the scrapes and 50% of the biopsies were positive for HPV infection. Scrapes detected more HPV 18 (10% vs. 2%, P = < 0.05) and HPV 31 (7% vs. 3%, not significant) than did the biopsies, but biopsies detected more HPV 16 (42% vs. 33%, not significant). Comparison of the results for each patient revealed that the correlation between scrapes and biopsies was not very close: only 34 patients were HPV‐positive by both sampling methods, whereas 67 were positive if the results were combined. This report discusses the implications of these findings.