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Enhancement of Inoue‐Melnick virus synthesis by 5‐bromodeoxyuridine in human meningioma (MG‐1) cells
Author(s) -
Nishibe Y.,
Inoue Y. K.,
Kimura T.,
Hase A.
Publication year - 1989
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890270304
Subject(s) - bromodeoxyuridine , virus , clone (java method) , virology , titer , cell culture , biology , microbiology and biotechnology , cytoplasm , staining , chemistry , dna , biochemistry , cell growth , genetics
The authors found remarkable enhancement of Inoue‐Melnick virus (IMV) synthesis by 5‐bromodeoxyuridine (BUDR) in human meningioma (MG‐1) cells, a virus‐producer line of type 1 IMV. Treatment with BUDR resulted in rapid and abundant synthesis of infectious IMV in MG‐1 cells. The titer of the cell‐associated virus in treated cells increased approximately 6.0 log 10 compared with that in untreated cells. Immunofluorescent antibody tests revealed that IMV‐associated late antigen was induced by BUDR in the cytoplasm of approximately 50% of treated cells. A clonal difference was also found in the enhancement of BUDR on the IMV synthesis in MG‐1 cells. The most remarkable enhancing effect of BUDR was observed in the clone C line, and a herpes‐type virus was detected by negative‐staining electron microscopy in the culture fluid of the clone C treated with BUDR.