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Recovery of antigenically reactive HIV‐2 cores
Author(s) -
Chrystie Ian L.,
Almeida June D.
Publication year - 1989
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890270303
Subject(s) - antigenicity , glutaraldehyde , monoclonal antibody , virology , antiserum , human immunodeficiency virus (hiv) , virus , antibody , biology , negative stain , immune system , electron microscope , chemistry , immunology , chromatography , physics , optics
Negative staining studies of human immunodeficiency virus (HIV) have been hampered by the fragile nature of the particles. Although detergent treatment is capable of releasing cores from HIV‐2 particles, these are unstable and do not retain morphological integrity. Addition of glutaraldehyde will stabilise these structures but, if used at too high a concentration, will destroy their antigenicity. This study shows that if both detergent and glutaraldehyde are used in correct proportions, antigenically reactive cores can be recovered from HIV‐2 cell cultures. More specifically we show that a mixture of 0.1% Nonidet P40 and 0.1% glutaraldehyde produces preparations of HIV‐2 cores that are suitable for immune electron microscopy. These cores reacted positively, that is, formed immune complexes, with both human HIV‐2 antisera and a mouse monoclonal antibody that, although directed against p24 (HIV‐1), reacts also with p25 (HIV‐2).