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Antigenic variation of human RSV strains isolated in Japan
Author(s) -
Tsutsumi Hiroyuki,
Nagai Kazushige,
Suga Kazuhiro,
Chiba Yasuo,
Chiba Shunzo,
Tsugawa Satoshi,
Ogra Pearay L.
Publication year - 1989
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890270211
Subject(s) - epitope , virology , monoclonal antibody , biology , antigen , antibody , antigenic variation , phosphoprotein , virus , nucleoprotein , paramyxoviridae , glycoprotein , serology , microbiology and biotechnology , viral disease , immunology , gene , genetics
Antigenic variations of respiratory syncytial virus (RSV) strains were analyzed using a collection of nine, seven, two, and one monoclonal antibodies (MAbs), respectively, raised against the fusion protein (F), large glycoprotein (G), nucleoprotein (NP), and phosphoprotein (P) components of the Long strain of RSV. Competitive binding assay by these MAbs demonstrated eight, four, and two distinct epitopes on F, G, and NP components, respectively. Comparison of prototype Long with ten field strains isolated in Sapporo, Japan, during a 9‐year period from 1980 to 1988 by radioimmunoprecipitation (RIP), immunofluorescence (IF), and enzyme‐linked immunosorbent assay (ELISA) test revealed four different patterns of reaction to these MAbs. Thus, prototype Long reacted to all 19 MAbs. Six field strains have shown a different reactivity to one of nine anti‐F and to one of seven anti‐G antibodies (subgroup A). Three of the remaining isolates failed to react with three of nine anti‐F and with all of seven anti‐G antibodies (subgroup B). One strain (58–104) isolated in 1983 was similar to subgroup A except for a lack of reaction with two anti‐G antibodies. All field strains reacted with two anti‐NP and one anti‐P antibodies. The numbers of altered epitopes in subgroup A were 1/8 and 1/4; in subgroup B, 3/8 and 4/4; and in 58–104, 1/8 and 2/4 on the F and G components, respectively. No other variations have been observed among field isolates tested.