Premium
False‐positive sera do not react with human immunodeficiency virus (HIV) Gag ‐encoded recombinant antigen
Author(s) -
Bukrinsky M. I.,
Syrtsev V. A.,
Popov S. A.,
Barsov E. V.,
Chaplinskas S. A.,
Karamov E. V.
Publication year - 1989
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890270115
Subject(s) - recombinant dna , virology , epitope , group specific antigen , antigen , western blot , virus , biology , microbiology and biotechnology , recombinant virus , human immunodeficiency virus (hiv) , hiv antigens , lentivirus , viral disease , gene , immunology , biochemistry
Ten sera from healthy blood donors positive by enzyme‐linked immunoadsorbent assay (ELISA) were studied by immunoblot assay using natural and recombinant proteins. They interacted only with p17 or p24 proteins but were nonreactive with a recombinant protein (RP 50), which carries antigenic determinants to p17 and p24. Reactions were not blocked by preincubation of sera with genetically engineered p17 and p24 or purified viral p24, indicating that some new epitopes were formed during the Western blot procedure. Recombinant gag ‐encoded protein is required for confirmation of human immunodeficiency virus (HIV) seropositivity.