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Nucleic acid spot hybridization with nonradioactive labeled probes in screening for human papillomavirus DNA sequences
Author(s) -
Melki Ronald,
Khoury Bernadette,
Catalan Francois
Publication year - 1988
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890260205
Subject(s) - dna , nucleic acid , nucleic acid thermodynamics , hybridization probe , nitrocellulose , microbiology and biotechnology , dna–dna hybridization , biology , human papillomavirus , molecular probe , polymerase chain reaction , in situ hybridization , virology , base sequence , gene , biochemistry , medicine , messenger rna , membrane
We examined 161 human tissue samples using the spot hybridization technique with nonradioactive labeled DNA probes of human papillomavirus (HPV). Whole cells were spotted on nitrocellulose filters; DNA of the cells was denatured and fixed to the filter. Then the DNA spots were hybridized to nonradioactive labeled DNA and monitored by a sandwich immunoenzymatic reaction. This technique is simple, sensitive, specific, requires no special equipment, and can be used in clinical settings. HPV DNA was found in 92% of samples in which, on the basis of histologic and colposcopic criteria, its presence was suspected, as well as in 31 samples where it was not suspected.