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Status of hepatitis B virus DNA in hepatocellular carcinoma: A study based on paired tumor and nontumor liver tissues
Author(s) -
Lai MingYang,
Chen DingShinn,
Chen PeiJer,
Lee ShengChung,
Sheu JinChuan,
Huang GuanTarng,
Wei TaCheng,
Lee ChueShue,
Yu SenChang,
Hsu HeyChi,
Sung JueiLow
Publication year - 1988
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890250302
Subject(s) - hepatocellular carcinoma , hbsag , southern blot , hepatitis b virus , hbeag , virology , hepadnaviridae , biology , virus , hepatitis b , medicine , dna , cancer research , genetics
To investigate the hepatitis B virus (HBV) DNA status in the liver when hepatocellular carcinoma (HCC) has developed, 35 paired nontumorous and tumorous liver tissues from 27 hepatitis B surface antigen (HBsAg)‐seropositive and 8 HBsAg‐negative patients with HCC were studied by Southern blot analysis. The hybridization patterns of HBV DNA were different in the nontumor and tumor parts in 26 (96.3%) of the 27 HBsAg‐positive patients. In the nontumor parts, integration of HBV DNA into the host genome was significantly less when compared to the tumor parts (15/27 vs. 25/27, P < 0.05), whereas free replicative viral forms were significantly more frequent (17/27 vs. 7/27). The integrated HBV DNA in the nontumor parts showed discrete band patterns in the majority of cases (13/15). Hepatitis B e antigen (HBeAg) was significantly associated with the expression of free replicative forms of HBV DNA in the tumor tissues. An integrated HBV DNA sequence was detected in the tumor part of one HBsAg‐negative patient, but not in her nontumor counterpart. Our observation that discrete integrated HBV DNAs are present in the nontumor part, representing subclinical clonal expansion that precedes the development of HCC, suggests the risk of future new tumor growth from these cell clones.