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Diagnosis of fetal rubella infection by nucleic acid hybridization
Author(s) -
HoTerry L.,
Terry G. M.,
Londesborough P.,
Rees K. R.,
Wielaard F.,
Denissen A.
Publication year - 1988
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890240206
Subject(s) - rubella virus , rubella , nucleic acid , virology , virus isolation , isolation (microbiology) , virus , fetus , nucleic acid thermodynamics , biology , togaviridae , dna , pregnancy , microbiology and biotechnology , genetics , vaccination , base sequence , measles
The efficacy of nucleic acid hybridization for the diagnosis of rubella infection in experimental and clinical materials was compared with immunoblot and virus isolation techniques. Our results showed that nucleic acid hybridization is specific and rapid but gives false‐negative results when compared with conventional virus isolation in some experimental although not in clinical materials so far examined. For this reason, a failure to demonstrate rubella virus in fetal specimens by this method alone cannot yet be taken as a sole criterion for ruling out fetal rubella infection.