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Detection of mononuclear cells expressing hepatitis B virus in peripheral blood from HBsAg positive and negative patients by in situ hybridisation
Author(s) -
Hadchouel M.,
Pasquinelli C.,
Fournier J. G.,
Hugon R. N.,
Scotto J.,
Bernard O.,
Brechot C.
Publication year - 1988
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890240105
Subject(s) - peripheral blood mononuclear cell , hbsag , hbeag , virology , hepatitis b virus , virus , rna , in situ hybridization , medicine , hepatitis b , hepadnaviridae , biology , immunology , microbiology and biotechnology , in vitro , messenger rna , gene , biochemistry
In situ hybridisation was used to detect RNA of Hepatitis B virus (HBV) in peripheral mononuclear blood cells. Presence of HBV‐RNA was detected in 10/17 HBsAg positive HBeAg positive patients, in 8/18 HBsAg positive HBeAg negative patients, in 1/3 anti‐HBs positive subjects. Four control subjects were negative. The frequency of labelled cells varied from 1 to 10% of mononuclear cells. These results indicate that the DNA detected so far in mononuclear blood cells may be transcriptionally active.