Premium
Detection of antibody to viral proteins following primary infection with herpes simplex virus
Author(s) -
Simmonds P.,
Smith I. W.,
Peutherer J. F.
Publication year - 1987
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890230212
Subject(s) - virology , herpes simplex virus , antibody , glycoprotein , antigen , biology , capsid , virus , alphaherpesvirinae , viral envelope , herpesviridae , viral disease , immunology , microbiology and biotechnology
Sera from patients with primary genital infection with herpes simplex virus (HSV) were tested by immunoblotting (IB) and radioimmunoprecipitation (RIP) analysis to determine the protein targets of antibody elicited by infection. The two tests detected antibody to different antigens: IB primarily detected reactivity with p40, a phosphorylated capsid protein, and RIP detected antibody to glycoprotein B, a viral envelope component. Furthermore, RIP detected antibody at an earlier stage of infection then IB. A nondenaturing version of IB was developed and used to investigate the role that the solubilisation of antigen plays in the sensitivity of each test for antibodies with different specificities.