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Cloning and characterisation of a delta virus cDNA Sequence derived from a human source
Author(s) -
Saldanha J. A.,
Thomas H. C.,
Monjardino J. P.
Publication year - 1987
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890220405
Subject(s) - viroid , complementary dna , cloning (programming) , virology , biology , virus , nucleic acid sequence , coding region , sequence analysis , open reading frame , sequence (biology) , genetics , computational biology , peptide sequence , plant virus , dna , gene , computer science , programming language
We report the extraction of delta virus RNA from the serum of a delta‐virus‐infected patient and the subsequent cloning and analysis of a 380‐nucleotide‐long cDNA (D380). The nucleotide sequence of D380 shows overall differences of approximately 20% when compared with previously published sequences and docs not include the viroid consensus sequence previously reported (Wang et al: Nature 323:508‐514, 1986). A potentially coding open reading frame extending over the whole length of the D.380 has been identified. Our results demonstrate the existence of genetic heterogeneity amongst different delta virus isolates.