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Production and characterisation of a human monoclonal antibody to cytomegalovirus and its use in an early nuclear fluorescence assay
Author(s) -
Sutherland S.,
Crawford D. H.,
Wilson S. A.,
Morgan B.,
Azim T.,
Huehns E. R.
Publication year - 1987
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890220308
Subject(s) - monoclonal antibody , virology , cytomegalovirus , peripheral blood mononuclear cell , human cytomegalovirus , antigen , biology , antibody , monoclonal , betaherpesvirinae , herpesviridae , microbiology and biotechnology , viral disease , immunology , virus , in vitro , genetics
A human monoclonal antibody to cytomegalovirus (CMV) was produced by transforming peripheral blood mononuclear cells of a patient with recent CMV infection. It is directed against a late antigen located in the nucleus of CMV infected fibroblasts at 24‐72 hours post infection and immuneprecipitates 65K and 48K proteins from 35 S‐labelled CMV infected cells. Results of its use in an early nuclear fluorescence assay for rapid diagnosis are presented.