Premium
Inhibition of hepatitis B virus DNA polymerase by 3′‐azido‐3′‐deoxythymidine triphosphate but not by its threo analog
Author(s) -
Nordenfelt E.,
Löfgren Bengt,
Chattopadhyaya Jyoti,
Öberg Bo
Publication year - 1987
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890220306
Subject(s) - dna polymerase , polymerase , hepatitis b virus dna polymerase , microbiology and biotechnology , virology , hepatitis b virus , dna , chemistry , dna synthesis , nucleotidyltransferase , virus , biology , biochemistry , rna , gene
An inhibitory effect of 3′‐azido‐3′‐deoxythymidine triphosphate on hepatitis B virus DNA polymerase was found. No effect was seen by its threo analog. The effect was detected at and above a concentration of 0.05 μM. The inhibition of DNA polymerase activity was the same in ten different strains of HBV. The mechanism was shown to be competitive, with an inhibition constant (Ki) of ˜0.04 μM, and the Km value for dTTP was 0.1 μM.