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Development of a plaque assay for a cytopathic, rapidly replicating isolate of hepatitis A virus
Author(s) -
Cromeans Theresa,
Sobsey Mark D.,
Fields Howard A.
Publication year - 1987
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890220107
Subject(s) - virology , cytopathic effect , virus quantification , polyclonal antibodies , antiserum , hepatitis a virus , biology , virus , cell culture , monoclonal antibody , monoclonal , viral replication , microbiology and biotechnology , antibody , immunology , genetics
Most hepatitis A virus (HAV) replication in cell culture has been reported to be nonlytic and relatively slow. A rapidly replicating isolate of strain HM‐175 from persistently infected, serially passed cell cultures (pHM‐175) was found to induce a cytopathic effect. This observation allowed the development of a classic plaque assay for pHM‐175 in FRhK‐4 cells. The plaques were neutralized by polyclonal and monoclonal antisera to HAV.