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Cytopathology, plaque assay, and heat inactivation of hepatitis A virus strain HM175
Author(s) -
Anderson David A.
Publication year - 1987
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890220106
Subject(s) - virology , virus quantification , virus , subculture (biology) , cytopathology , poliovirus , cytopathic effect , biology , microbiology and biotechnology , strain (injury) , hepatitis a virus , cytology , genetics , anatomy
Hepatitis A virus (HAV) strain HM175 derived after repeated subculture of persistently infected B‐SC‐I cells caused a specific cytopathic effect upon acute infection of B‐SC‐1 cells. The virus formed visible plaques on B‐SC‐I cell monolayers after 9 to 14 days of incubation at 34°C. and virus can therefore be titrated by plaque assay. Virus could be re‐isolated from plaques of infected cells. which allows the clonal isolation of HAV variants. The stability of a plaquepurified variant of HAV at elevated temperatures exceeded that of poliovirus type 1. but this variant is less stable than previously reported strains of HAV.