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Analysis by immunoblotting of human cytomegalovirus antibody in sera of renal transplant recipient
Author(s) -
Shimokawa Keiko,
Murayama Tsugiya,
Ishida Shigeo,
Furukawa Toru
Publication year - 1987
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890210406
Subject(s) - human cytomegalovirus , cytomegalovirus , virology , antibody , renal transplant , betaherpesvirinae , herpesviridae , biology , immunology , virus , medicine , viral disease , kidney , genetics
Human cytomegalovirus‐infected cell polypeptides were immunoreacted by sera of renal transplant recipients and compared with those reactive with sera of healthy adult donors by means of the Western immunoblotting technique. At least 15 polypeptides with molecular weights of 155K, 123K, 102K, 89K, 79K, 71K, 65K, 60K, 55K, 50K, 46K, 42K, 38K, 33K, and 28K were immunoreacted. Sera obtained serially from renal transplant recipients reacted with most of these polypeptides and reacted more frequently and intensely with the smaller polypeptide species such as 38K, 33K, and 28K, compared with sera of healthy seropositive adults. The implications of these findings are discussed.