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Cloning and characterization of BK virus‐related DNA sequences from normal and neoplastic human tissues
Author(s) -
Knepper Janice E.,
Dimayorca Giampiero
Publication year - 1987
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890210313
Subject(s) - papovavirus , biology , restriction enzyme , bk virus , dna , microbiology and biotechnology , in vitro recombination , molecular cloning , cloning (programming) , clone (java method) , genome , virology , restriction map , virus , genetics , gene , kidney , complementary dna , nucleic acid sequence , kidney transplantation , computer science , programming language
DNA homologous to that of the known papovavirus BK was cloned from the high molecular weight DNA of two human tissues: a normal liver and a kidney carcinoma. The clone isolated from human liver consisted of DNA indistinguishable from prototype BK by restriction enzyme analysis that used ten different enzymes. The DNA cloned from the human carcinoma of the kidney was subject to rearrangement in recombination‐deficient bacteria, and exhibited a deletion of a small segment of DNA localized to the BK late region. Restriction fragments representing the BK origin and promoter regions are overrepresented in the tumorderived clone. The possible significance of retrieval of defective viral genomes from tumor tissues is discussed.