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Detection of serum antibody responses to RIT 4237 rotavirus vaccine by ELISA and neutralization assays
Author(s) -
Delem A.,
Vesikari T.
Publication year - 1987
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890210306
Subject(s) - seroconversion , virology , serology , antibody , neutralization , rotavirus , virus , immunoglobulin m , medicine , immunoglobulin g , biology , immunology
Immunoglobulin class‐specific determination by ELISA and a virus neutralization test (NT) were compared for the detection of the serum antibody responses after vaccination of infants with the RIT 4237 rotavirus vaccine (of bovine origin). A capture method was applied for specific IgM determination by ELISA because of its greater sensitivity and reproducibility over the conventional ELISA‐IgM test. NT was improved through the use of an enzyme‐labelled antibody for the detection of non‐neutralized virus. In 6–12‐month‐old children, the most sensitive single test to detect an antibody response was the ELISA‐IgM capture method, but the combination of ELISA‐IgM and IgG tests or ELISA‐IgM and NT detected the highest seroconversion rate of 79%. In newborn infants high levels of maternal antibody made the ELISA‐IgG test unsuitable. ELISA‐IgM gave a response rate of 31%, but NT with homologous virus was the most sensitive indicator of a serological response to the vaccine in this age group, yielding a 45% response rate.