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Presence of episomal and integrated human papillomavirus DNA sequences in cervical carcinoma
Author(s) -
Choo KongBung,
Pan ChaoChih,
Liu MingSun,
Han ShouHwa,
Ng HeungTat,
Chen CyrePing,
Lee YiNan,
Chao ChungFaye,
Meng ChingLeung,
Yeh MingYang
Publication year - 1987
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890210202
Subject(s) - biology , virology , southern blot , human papillomavirus , cervix , cervical carcinoma , genome , virus , dna , carcinoma , microbiology and biotechnology , cervical cancer , genetics , gene , cancer , medicine
Thirty surgical samples of squamous cell carcinoma of the cervix obtained from Chinese women were analysed for the presence of human papillomavirus (HPV) types 16 and 18 using Southern blot hybridization procedure. HPV16 was detected in 53% while HPV18 was found in only 6% of the samples analyzed. When compared with other reports, variation in the geographic distribution of these two HPV types in association with cervical carcinoma is noted. Thirty‐seven and a half percent of the HPV16‐positive samples contained this HPV type in episomal form and an equal number in cellular DNA‐integrated form. The simultaneous presence of both episomal and integrated forms was found in the remaining 25 % of the positive samples. The two HPV18‐positive cases harbored only episomal viral genome and were not superinfected by HPV16. Analysis of the HPV16. integration samples showed that single integration events had probably occurred and some of the viral sequences had been lost on or subsequent to integration.