z-logo
Premium
Estimation of the B Lymphocyte Precursor Frequencies to Herpes Simplex Type 1 Glycoproteins by a Limiting Dilution Assay
Author(s) -
Turtinen Lloyd W.,
Lathey Janet L.,
Rouse Barry T.
Publication year - 1986
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890200408
Subject(s) - glycoprotein , titer , antibody , virology , herpes simplex virus , microbiology and biotechnology , herpesvirus glycoprotein b , biology , lymphocyte , immunization , neutralizing antibody , chemistry , immunology , virus , viral replication , viral entry
The precursor frequency of B lymphocytes from Balb/c mice producing HSV‐1 glycoprotein B (gB), glycoprotein C (gC), and glycoprotein D (gD) antibody was determined by limiting dilution analysis under conditions to detect antibody from the clonal progeny of a single B cell precursor. In spleens of naive mice the average gC frequency was 1/48,917 ± 550, while gD was 1/73,330 ± 15,898, and gB frequency was in excess of 1/1OO,ooO. Immunization with live HSV‐1 (KOS) increased the B cell frequencies of all three glycoproteins to approximately 1:3,000; however, the serum gB antibody ELISA titer was fivefold higher than gC or gD.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here