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Estimation of the B Lymphocyte Precursor Frequencies to Herpes Simplex Type 1 Glycoproteins by a Limiting Dilution Assay
Author(s) -
Turtinen Lloyd W.,
Lathey Janet L.,
Rouse Barry T.
Publication year - 1986
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890200408
Subject(s) - glycoprotein , titer , antibody , virology , herpes simplex virus , microbiology and biotechnology , herpesvirus glycoprotein b , biology , lymphocyte , immunization , neutralizing antibody , chemistry , immunology , virus , viral replication , viral entry
The precursor frequency of B lymphocytes from Balb/c mice producing HSV‐1 glycoprotein B (gB), glycoprotein C (gC), and glycoprotein D (gD) antibody was determined by limiting dilution analysis under conditions to detect antibody from the clonal progeny of a single B cell precursor. In spleens of naive mice the average gC frequency was 1/48,917 ± 550, while gD was 1/73,330 ± 15,898, and gB frequency was in excess of 1/1OO,ooO. Immunization with live HSV‐1 (KOS) increased the B cell frequencies of all three glycoproteins to approximately 1:3,000; however, the serum gB antibody ELISA titer was fivefold higher than gC or gD.