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Use of N‐acetylethyleneimine [AEI] for the inactivation of semliki forest virus in vitro
Author(s) -
Amor Sandra,
Webb H. E.
Publication year - 1986
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890190409
Subject(s) - semliki forest virus , virology , antigenicity , virus , biology , vero cell , strain (injury) , in vitro , alphavirus , inoculation , microbiology and biotechnology , antibody , immunology , rna , biochemistry , anatomy , gene
N‐Acetylethyleneimine (AEI) was used to inactivate the avirulent Togavirus Semliki Forest virus (A774 strain) grown in chick embryo, Vero, and brain cell cultures. The purity of the virus preparation affected the kinetics of inactivation. The rate of inactivation increased with a rise in temperature from 5 to 40°C and in concentration of AEI from 0.025 to 0.1%. The resultant vaccine was inoculated into adult mice to test its antigenicity and into suckling mice to test for the presence of infective virus. Semliki Forest virusspecific IgG was produced equal to that of mice given live virus, and mice were protected against the lethal SFV L10 strain. No suckling mice died, and the brains of the adult mice showed no pathology.