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Detection of early hemagglutination inhibitory antibodies to rubella virus by pretreatment of sera with streptococcal cells
Author(s) -
Kawano Kimiko,
Yamamoto Seigo,
Minamishima Yoichi
Publication year - 1986
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890190202
Subject(s) - rubella virus , rubella , virology , antibody , serology , hemagglutination , microbiology and biotechnology , hemagglutination assay , virus , immunology , biology , measles , vaccination , titer
Abstract A technique that is based on absorption of sera with streptococcal cells and hemagglutination inhibition (HI) was evaluated for its feasibility for serologic diagnosis of recent rubella. The mixture of AR1 and AW43 cells removes IgG and IgA from the kaolin‐treated sera, leaving IgM and a trace of IgA, probably oligomeric IgA. Consequently, after the absorption with streptococci, the HI antibodies are detectable exclusively in the early sera of patients with rubella. The streptococci (AR1 and AW43) have several advantages as the absorbent over the staphylucoccus (Cowan I) that has been used routinely.