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Development of a competitive enzyme‐linked immunosorbent assay for detecting cytomegalovirus antibody
Author(s) -
Wreghitt T. G.,
Hicks Joanna,
Gray J. J.,
O'Connor Candice
Publication year - 1986
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890180204
Subject(s) - complement fixation test , antibody , cytomegalovirus , virology , monoclonal antibody , horseradish peroxidase , competitive binding , human cytomegalovirus , enzyme , biology , microbiology and biotechnology , herpesviridae , serology , immunology , virus , viral disease , biochemistry , receptor
Abstract A competitive enzyme‐linked immunosorbent assay (ELISA) for detecting cytomegalovirus (CMV) antibody was developed. The competitive ELISA was five times more sensitive than the complement fixation test (CFT) and twice as sensitive as indirect ELISA. Testing of paired sera from cardiac transplant patients taken before and after transplantation showed good correlation between results of competitive and indirect ELISA and CFT. The competitive ELISA was more successful than CFT or indirect ELISA in detecting passively acquired antibody, but detection of CMV antibody by competitive ELISA immediately after primary CMV infection was unreliable, possibly because of the high affinity of the monoclonal antibody chosen for the horseradish peroxidase conjugate. However, competitive ELISA may well prove to be more suitable than indirect ELISA for detecting CMV antibody in blood donations.