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Detection of respiratory syncytial virus in nasopharyngeal secretions by a biotin‐avidin ELISA more sensitive than the fluorescent antibody technique
Author(s) -
Hornsleth Allan,
Friis Birgitte,
Krasilnikof Peter Andreas
Publication year - 1986
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890180203
Subject(s) - virology , biotin , avidin , antibody , paramyxoviridae , virus , respiratory system , biology , medicine , immunology , viral disease , genetics , anatomy
The presence of respiratory syncytial virus (RSV) was investigated by immunofluorescent antibody (IF) technique and by a biotin/avidin (BA) ELISA in 156 samples of nasopharyngeal secretions (NPS) obtained from infants and small children with acute respiratory disease. Of 70 RSV‐IF‐positive NPS, 68 were positive by BA‐ELISA. Of 86 RSV‐IF‐negative NPS, eight were positive by BA‐ELISA. BA‐ELISA could detect 0.5–1.0 ng RSV‐protein.