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Evaluation of a rapid passive hemagglutination assay for anti‐rubella antibody: Comparison to hemagglutination inhibition and a vaccine challenge study
Author(s) -
Safford John W.,
Abbott Glenda G.,
Deimler Colleen M.
Publication year - 1985
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890170304
Subject(s) - seroconversion , rubella , hemagglutination assay , rubella virus , virology , antibody , hemagglutination , vaccination , immunology , medicine , biology , microbiology and biotechnology , measles , titer
A rapid passive hemagglutination assay (Rubaquick™) was developed that detects antibody to rubella virus in serum specimens. The test result is read visually after an incubation period of 15–30 minutes. When compared with a hemagglutination inhibition assay, the Rubaquick assay results obtained from 1,470 sera were greater than 99% specific, sensitive, and accurate. Studies of 179 paired serum specimens obtained before and 27 days after rubella vaccination showed that if antibody was detectable by the Rubaquick assay in the prevaccination specimens, the vaccine induced a secondary response consisting of increasing IgG antibody reactivity in the absence of a positive IgM response. In contrast to the positive prevaccination specimens, a negative prevaccination result was associated with IgM antibody in 98 of the 133 postvaccination specimens. Seroconversion was noted in all cases in which the prevaccination specimen was negative by the Rubaquick assay.