Premium
Detection of BK virus IgM antibodies by two enzyme‐linked immunosorbent assays (ELISA) and a hemagglutination inhibition method
Author(s) -
Flaegstad Trond,
Traavik Terje
Publication year - 1985
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890170212
Subject(s) - antibody , virology , bk virus , hemagglutination assay , hemagglutination , antigen , immunoglobulin m , microbiology and biotechnology , virus , chemistry , biology , immunoglobulin g , immunology , titer , kidney transplantation , kidney , endocrinology
We have used an antigen solid‐phase enzyme‐linked immunosorbent assay (SP‐ELISA) and an IgM antibody capture ELISA (MACELISA) for detecting IgM antibodies to human polyomavirus BK (BKV). These tests were compared with the standard hemagglutination inhibition test (HAI) of IgM serum fractions following sucrose density gradient fractionation. The SP‐ and MACELISA were not influenced by concomitant BKV‐IgG, but high levels of both BKV‐IgG and rheumatoid factor could cause false positive results by SPELISA, but not by MACELISA. The MACELISA gave much higher positive to negative ratios than the SPELISA. The sensitivity and specificity of the two tests were high compared to the IgM‐HAI method. The sera could be tested in a single dilution (1:160), and thus the ELISA‐tests are useful for testing large numbers of sera.