Premium
Quantitative and qualitative detection of cytomegalovirus‐specific antibodies using two types of enzyme‐linked immunosorbent assay
Author(s) -
Kinane Kevin A.,
Hillary Irene B.
Publication year - 1985
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890160411
Subject(s) - complement fixation test , antibody , cytomegalovirus , virology , immunoglobulin g , antigen , human cytomegalovirus , immunology , microbiology and biotechnology , virus , biology , herpesviridae , serology , viral disease
An indirect ELISA and an inhibition ELISA were developed for the detection of cytomegalovirus (CMV)‐specific immunoglobulin G (IgG) and CMV‐specific total immunoglobulin, respectively. Both assays were more specific than the complement fixation (CF) test, and titres of positive sera were 660 times higher by IgG ELISA and 6 times higher by inhibition ELISA than titres by the CF test. Titres by IgG ELISA were reliably determined using the absorbance obtained at a single serum dilution of 1/1,000 in conjunction with a standard graph. Both ELISAs compared favourably with each other in sensitivity and specificity in determining CMV immune status. The inhibition ELISA, in particular, provides a simple and reliable method of screening sera, which requires no control antigen or predilution of sera. It should prove useful for large‐scale screening procedures, such as blood donor testing.