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Detection of BK virus antibodies measured by enzyme‐linked immunosorbent assay (ELISA) and two haemagglutination inhibition methods: A comparative study
Author(s) -
Flaegstad Trond,
Traavik Terje
Publication year - 1985
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890160408
Subject(s) - virology , antibody , haemagglutination inhibition , enzyme , hemagglutination , virus , hemagglutination assay , bk virus , chemistry , biology , microbiology and biotechnology , serology , immunology , biochemistry , titer , kidney transplantation , kidney , endocrinology
We developed a solid phase enzyme‐linked immunosorbent assay (ELISA) for detecting IgG antibodies to BK virus (BKV). This test was compared to two haemagglutination inhibition methods, one using receptor‐destroying enzyme and the other NaIO 4 to remove nonspecific inhibitors. The results show that this single dilution ELISA is a sensitive and specific test. The inherent advantages of this method make it an attractive alternative for the seroepidemiology of BKV.