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Inactivation of the hutchinson strain of non‐A, non‐B hepatitis virus by combined use of β‐propiolactone and ultraviolet irradiation
Author(s) -
Prince Alfred M.,
Stephan Wolfgang,
Dichtelmüller Herbert,
Brotman Betsy,
Huima Tellervo
Publication year - 1985
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890160204
Subject(s) - virology , virus , hepatitis a virus , virus strain , hepatitis virus , ultraviolet , ultraviolet irradiation , hepatitis , strain (injury) , inoculation , ultraviolet light , chemistry , irradiation , biology , microbiology and biotechnology , medicine , immunology , materials science , physics , optoelectronics , photochemistry , nuclear physics
A b̃‐propiolactone/ultraviolet irradiation procedure (βPL/UV) has been evaluated for its ability to inactivate 30,000 chimpanzee infectious doses of the Hutchinson strain of non‐A, non‐B (NANB) virus. The chimpanzees were inoculated with plasma to which this dose of the titrated virus had been added prior to application of the βPL/UV process in accordance with a procedure used for licensed blood derivatives in Germany. Neither animal developed hepatitis. When subsequently challenged with the same contaminated plasma, which had not been sterilized, both animals promptly developed typical NANB hepatitis. This study extends the high (∼10 7 ‐fold) process efficiency of the βPL/UV procedure previously reported for hepatitis B virus to a blood‐borne NANB virus.