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Early and late antigens of human cytomegalovirus:Electroimmunodiffusion assay of numbers, relationships, and reactivities with donor sera
Author(s) -
Sweet George H.,
Bryant Steven A.,
Tegtmeier Gary E.,
Beneke Janet S.,
Bayer William L.
Publication year - 1985
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890150206
Subject(s) - complement fixation test , antigen , virology , antibody , cytomegalovirus , immunofluorescence , virus , biology , indirect immunofluorescence , immunology , serology , herpesviridae , viral disease
Early antigens (EA) of human cytomegalovirus extracted from cytosine arabinoside‐blocked cells infected with 0.01–20 infectious units (IU)/cell were assayed with human serum by electroimmunodiffusion (EID). The number of detectable EA types increased from one to eight as the IU/cell was raised from 0.01 to 10. There was no increase in the number of EA with further increases in IU/cell, with prolonged culture, or when detergent was included in the extraction buffer. At least five of the eight EA gave reactions of identity with late‐time antigens (LTA) extracted from unblocked cells at late times postinfection. In studies on a panel of sera from donors who were excreting virus and donors who were not, EID was as sensitive as conventional techniques (complement fixation and indirect hernagglutination for LTA, indirect immunofluorescence for EA) in detection of both types of antibodies from excretors but less sensitive in not detecting low levels of the antibodies in some of the sera from nonexcretors. No consistent relationships were observed between donor virological status and the numbers or types of antibodies to EA and LTA.