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The effects of human cytomegalovirus challenge in vitro on subpopulations of T cells from seronegative donors
Author(s) -
Sing Garwin Kim,
Garnett Helen M.
Publication year - 1984
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890140409
Subject(s) - monoclonal antibody , virology , in vitro , suppressor , cytomegalovirus , indirect immunofluorescence , biology , immunofluorescence , virus , mixed lymphocyte reaction , t lymphocyte , immunology , antibody , microbiology and biotechnology , herpesviridae , t cell , viral disease , immune system , biochemistry , genetics , cancer
Purifed T cells from seronegative donors were challenged with human cytomegalovirus (CMV) and assayed for response to the mitogen phytohemagglutinin (PHA) administered at various times after virus challenge. Cells were hyporesponsive to PHA added up to 6 h postinfection, while cells challenged with ultraviolet (UV)‐inactivated CMV showed normal PHA responses. T‐cell populations showed a complete inversion of helper/suppressor ratios by 6 h of infection, as determined by indirect immunofluorescence using the monoclonal antibodies OKT4 and OKT8. This increased proportion of suppressor cells was functionally demonstrable in an autologous one‐way mixed lymphocyte reaction (AMLR).