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DNA of 21 clinical cytomegalovirus strains detected by hybridization to cloned DNA fragments of laboratory strain AD‐169
Author(s) -
Chou Sunwen,
Roark Linda,
Merigan Thomas C.
Publication year - 1984
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890140310
Subject(s) - virology , cytomegalovirus , biology , dna , hybridization probe , microbiology and biotechnology , strain (injury) , genome , nucleic acid thermodynamics , dna–dna hybridization , herpesviridae , virus , gene , viral disease , genetics , base sequence , anatomy
Nine distinct DNA probes prepared from cloned DNA fragments of the cytomegalovirus (CMV) strain AD‐169, each representing a relatively small portion of the total genome, were able to detect all 21 epidemiologically distinct clinical isolates of CMV that had been passaged in tissue culture. All probes were of comparable sensitivity in the detection of CMV, and under the hybridization conditions used, no probe gave an unusually high background with uninfected host cells.