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Isolation and characterization of liver‐derived hepatitis B e antigen
Author(s) -
Budkowska Agata,
Petit MarieAnne,
Karwowska Sylwia,
Roszuk Danuta
Publication year - 1984
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890130309
Subject(s) - hbeag , size exclusion chromatography , antigen , virology , chemistry , hepatitis b virus , dimer , hepatitis b , microbiology and biotechnology , virus , chromatography , biology , biochemistry , enzyme , hbsag , immunology , organic chemistry
Two subpopulations of hepatitis B e antigen (HBeAg) were isolated from a human liver infected with hepatitis B virus. HBeAg extracted from liver homogenate subsequent to treatment with buffered 3 M NaSCN or 0.5 M MgCl 2 banded at the density of 1.13 g/cm 3 in CsCl and was polydispersed on gel filtration. In contrast, HBeAg released with phosphate‐buffered saline (PBS) was detected mainly at a density of 1.20 g/cm 3 in a CsCl gradient and consisted of low molecular weight species on gel chromatography. Polypeptides of 40,000 and 45,000 daltons were found in NaSCN and PBS‐released HBeAg preparations, respectively. The results are interpreted as suggestive that liver HBeAg is a dimer of the major core particle polypeptide in different physicochemical forms.