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In vitro neutralization of HSV‐2: Inhibition by binding of normal IgG and purified Fc to virion Fc receptor (FcR)
Author(s) -
Dowler Kenneth W.,
Veltri Robert W.
Publication year - 1984
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890130307
Subject(s) - neutralization , antiserum , infectivity , virology , in vitro , chemistry , antibody , receptor , microbiology and biotechnology , virus , fc receptor , biology , biochemistry , immunology
Abstract We designed experiments to assess the effects of binding of the HSV‐2 Fc receptor (FcR) to purified rabbit nonimmune IgG and purified Fc. Purified Fc (75 μg) or nonimmune IgG (100 μg) when bound to HSV‐2 did not reduce infectivity but did protect the virions against thermal inactivation at 37°C. However, preincubation of these two reagents with HSV‐2 virions significantly protected against neutralization by specific anti‐HSV‐2, F(ab') 2 purified rabbit antiserum. The blockage of neutralization and protection against thermal inactivation afforded by FcR‐Fc interaction on HSV‐2 virions provide a tenable mechanism to explain viral persistence in an immune host.