Premium
Hemagglutination by human rotavirus strains
Author(s) -
Kitaoka Setsuko,
Suzuki Hiroshi,
Numazaki Toshio,
Sato Tetsuo,
Konno Tasuke,
Ebina Takusaburo,
Ishida Nakao,
Nakagomi Osamu,
Nakagomi Toyoko
Publication year - 1984
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890130303
Subject(s) - hemagglutination , virology , infectivity , titer , rotavirus , antiserum , hemagglutination assay , biology , trypsin , microbiology and biotechnology , centrifugation , neutralization , antigen , virus , biochemistry , enzyme , immunology
Human rotavirus isolates, KUN, MO, and Wa strains were found to agglutinate erythrocytes of the day‐old chicken and adult goose, optimally at pH 6.6. Only those fractions containing double‐shelled rotavirus particles isolated by isopycnic centrifugation in cesium chloride had hemagglutinating activity. Trypsin treatment decreased the hemagglutination titer of human rotavirus strains and conversely increased their infectivity. Hemagglutination inhibition tests with antisera against type‐specific antigens demonstrable by neutralization showed no type specificity.