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Replication of human rotavirus in cell culture
Author(s) -
Birch Christopher J.,
Rodger Suzanne M.,
Marshall John A.,
Gust Ian D.
Publication year - 1983
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890110307
Subject(s) - virology , rotavirus , trypsin , cytopathic effect , immunofluorescence , virus , biology , cell culture , reoviridae , viral replication , microbiology and biotechnology , replication (statistics) , antibody , enzyme , immunology , genetics , biochemistry
Nine strains of human rotavirus were adapted to growth in CV‐1 and/or MA‐104 cells following pretreatment of virus with trypsin, incorporation of trypsin into culture medium, and use of roller cultures. Immunofluorescence was the most reliable method for the detection of virus replication, although characteristic cytopathic effects were produced sporadically by most isolates. Virus could be readily detected in supernatant fluids of cell cultures and in cell sections by electron microscopy.